Citrate agar results
Welcome to Microbugz. Citrate Test. Simmons citrate agar tests the ability of organisms to utilize citrate as a carbon source. Simmons citrate agar contains sodium citrate as the sole source of carbon, ammonium dihydrogen phosphate as the sole source of nitrogen, other nutrients, and the pH indicator bromthymol blue.Deoxycholate Citrate Agar is a modification of Deoxycholate Agar formulated by Leifson. DCA is selective for enteric pathogens owing to increased concentrations of both citrate and deoxycholate salts. Sodium deoxycholate at pH 7. 3 to 7. 5 is inhibitory for gram positive bacteria. citrate agar results
Oct 13, 2018 Simmons citrate agar is a selective and differential medium that tests for an organisms ability to use citrate as a sole carbon source and ammonium ions as the sole nitrogen source. It is used for differentiating gramnegative bacteria on the basis of citrate utilization.
Expected results in citrate utilization test: Citrate negative: trace or no growth will be visible. No color change will occur; the medium will remain the deep forest green color of the uninoculated agar. Only bacteria that can utilize citrate as the sole carbon and energy source will be able to grow on the Simmons citrate medium, Jun 11, 2018 Principle of Simmons Citrate Agar. Simmons Citrate agar is used to test an organisms ability to utilize citrate as a source of energy. Ammonium Dihydrogen Phosphate is the sole source of nitrogen. Dipotassium Phosphate acts as a buffer. Sodium Chloride maintains thecitrate agar results SUMMARY. (9) Simmons Citrate Agar contains ammonium dihydrogen phosphate, which supplies the only source of nitrogen, and sodium citrate, which serves as the sole source of carbon. Organisms capable of utilizing ammonium dihydrogen phosphate and citrate will grow unrestricted on this medium. Bromothymol blue acts as a pH indicator,